• br Material and methods br Acknowledgments We are

  2018-10-22

  

  Material and methods Acknowledgments We are grateful to D. Trono (Lausanne, Switzerland) for the plasmid pMD2.G and psPAX2. We thank Brigitte Jenewein for her excellent technical assistance with the confocal laser-scanning microscopy and the flow cytometry analysis. This work was supported by

• To provide a comprehensive landscape in

  2018-10-22

  

  To provide a comprehensive landscape in human hematopoiesis, we studied NF-E2 gene expression throughout the hematopoietic system using HemaExplorer (Bagger et al., 2013) and confirmed by RT-PCR that it was also expressed in the stem cell compartment (Figure 1A). Thereafter, we knocked NF-E2 down in

• Several transcripts that exhibited an expression change trig

  2018-10-22

  

  Several transcripts that exhibited an expression change triggered by T3 are promising candidates for being responsible for the observed increased cell extension growth of ENS cells in vitro. The calcium dependent cell adhesion molecule cadherin 12 (Cdh12, N-cadherin, 5.6 fold upregulated), the gene

• Introduction Bone marrow BM derived

  2018-10-20

  

  Introduction Bone marrow (BM)-derived endothelial progenitor GW 610 Supplier (EPCs) circulate in peripheral blood and play an important role in the formation of new blood vessels (Asahara et al., 1997; Takahashi et al., 1999; Zhou et al., 2013; Li et al., 2011). This process occurs in response to a

• Discussion For several years BMSCs have been

  2018-10-20

  

  Discussion For several years, BMSCs have been largely studied and used as a new therapeutic tool for clinical applications because of their multipotent properties (Dimarino et al., 2013). Osteonecrosis is a serious complication of SCD (Milner et al., 1991). To treat this bone disease, we suggest a

• Following fluorescence activated cell sorting FACS for dToma

  2018-10-20

  

  Following fluorescence activated cell sorting (FACS) for dTomato-reporter expression, HSPCs were maintained for ten days in liquid culture using StemSpan medium (StemCell Technologies, Grenoble, France) supplemented with 1% penicillin/streptomycin, 1% l-glutamine (Biochrom AG, Berlin, Germany) and t

• Introduction The vasculature is a multicellular system in

  2018-10-20

  

  Introduction The vasculature is a multicellular system in which each cell type plays an important and indispensible role in its function. The inner lining of endothelial cells (ECs), which are in direct contact with the blood, is surrounded and supported by perivascular cells—either vascular smooth

• br Resource table Resource details

  2018-10-20

  

  Resource table. Resource details Macaca fascicularis iPS rad51 inhibitor ATCi-MF1 were generated from adult skin-derived fibroblasts by transduction with non-integrative Sendai viruses coding for the reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC (Ban et al. 2011; Zapata-Linares et al. 2016)

• Unlike the LMW FGF isoform which is

  2018-10-20

  

  Unlike the LMW FGF2 isoform, which is well-studied, the HMW isoforms of FGF2 are not nearly as well-understood, but recent reports have investigated the effects of HMW FGF2 signaling in various contexts. Localization of FGF2 isoforms can vary depending on cellular conditions and stimuli (Arese et al

• Multi electrode array approaches have been

  2018-10-20

  

  Multi-electrode array approaches have been proposed as a tool for detecting functional changes in electrically excitable cells, including neurons, exposed to drugs or toxins and allow use in high throughput studies (McConnell et al., 2012). Although there are a number of electrophysiological measure

• br Introduction Stem cells are essential for tissue

  2018-10-20

  

  Introduction Stem cells are essential for tissue formation, maintenance, and repair by achieving a timely balance between error-free replicative expansion and differentiation. This balance is frequently jeopardized in cancer and aging, leading to tissue pathology and functional decline (Blanpain

• br Experimental Procedures br Author Contributions

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction Bone marrow stromal cells (BMSCs, also called bone-marrow-derived mesenchymal stromal cells) are heterogeneous populations that likely contain varying levels of tripotent (osteogenic, adipogenic, and chondrogenic [

• In mammalian PGCs genetic knockout

  2018-10-20

  

  In mammalian PGCs, genetic knockout models and short-term PGC culture experiments have implicated the growth factors BMP4, LIF, SCF, retinoic acid, and FGF in early survival and proliferation (Dolci et al., 1991, 1993; Farini et al., 2005; Matsui et al., 1991). PGCs isolated from mammalian species c

• Several strategies have been recently developed

  2018-10-20

  

  Several strategies have been recently developed for CBC cell isolation via cell surface markers and fluorescence-activated cell sorting (FACS; Gracz et al., 2013; King et al., 2012; Merlos-Suarez et al., 2011; Wang et al., 2013). Although they represent considerable advances in the isolation of CBC

• br Experimental Procedures br Author Contributions br

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments This work is supported by national Portuguese funding through FCT, Fundação para a Ciência e a Tecnologia, project UID/BIM/04773/2013 CBMR, projects PEst-OE/EQB/LA0023/2013 and PTDC/SAU-ENB/111702/2009 to J.B., U01HL100408, NIH/NH

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