br Methods of survey data collection and analysis br

Methods of survey, data collection and analysis
Conflict of Interest
Acknowledgements
Data Data provided in this article correspond to the FASTQ files obtained after RNA sequencing of melanoma receptor tyrosine kinase from two mouse models, treated with scramble shRNA or Rack1-targeting shRNA.
Experimental design, materials and methods
Acknowledgements This work was funded by a grant from Institut National de la Recherche Agronomique (IPRM-RACK) (INRA- Crédits incitatifs du département de génétique animale) to GE.
Data This dataset extends the reproducibility of the recent published data [1]. BMDMs derived from WT and MKP-1-/- mice exhibit higher p38 and JNK activation in response to LPS (Fig. 1). HIF-1α expression is significantly increased at baseline and in response to LPS challenge in nuclear extracts of MKP-1 deficient BMDMs under normoxic condition as compared to WT BMDMs (Fig. 2). In nuclear extracts of MKP-1 deficient BMDMs expression of p300, a transcriptional coactivator that binds with HIF-1α at the promoter regions of targeted genes, is significantly increased at baseline and in response to LPS treatment (Fig. 2). LPS-induced HIF-1α expression is significantly inhibited by SB203850, a specific inhibitor of p38 but not by SP600125, a specific inhibitor of JNK (Fig. 3) LPS-induced IL-1β, TNF-α, and IL-6 production in MKP-1 deficient BMDMs are significantly inhibited by SB203850, a specific inhibitor of p38 MAPK (Fig. 4).
Experimental design, materials and methods
Acknowledgements
Data The data shown in Section 1.1 of this article provide detailed information on the veterinary clinics that participated in a recent survey of the prevalence of fecal shedding of Clostridium perfringens and C. difficile by dogs and cats which was carried out in the Madrid region (Spain) [1]. Furthermore, the demographic and clinical features of recruited animals are detailed in Section 1.2, and Section 1.3 provides extensive data on the genetic and phenotypic characteristics of recovered bacterial isolates.
Experimental design, materials and methods The owners of animals yielding a positive culture for C. difficile and/or C. perfringens were invited to participate in a follow-up survey performed four months after the first study (in March 2016). In this case, fecal swab samples and clinical information of animals was obtained as explained above. The microbiology procedures used for C. perfringens and C. difficile isolation from fecal samples, and the methods used for toxin profiling, PCR ribotyping, AFLP subtyping and in vitro antimicrobial susceptibility testing of recovered isolates are detailed in our previous publication [1].
Funding sources This work was supported by grant AGL2013-46116-R from the Spanish Ministry of Economy and Competitiveness. The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
Specifications Table
Value of the data
Data Table 1 shows the characteristics of the raw leachates. Figs. 1 and 2 depict the COD and colour removal at different leachate pHs respectively as a function of time. Fig. 3 shows the pseudo first-order kinetic plot for the leachate COD removal by the Cu/Mg/Al-chitosan-O3 system. Results for the economic evaluation of leachate treatment by Cu/Mg/Al-chitosan-O3 system are presented in Table 2.
Experimental design, materials and methods
Acknowledgements This dataset was technically assisted by the Bushehr University of Medical Sciences, Bushehr, Iran. Tehran Wastes Management Organization, Iran is also gratefully acknowledged for their collaborations during leachate sampling.
Data
Experimental design, materials and methods All experimental design, materials and methods were based on reported paper [1]. The scanning electron microscopy (SEM) images of the Au NWIs were obtained by FEI Nova 230 (EI Company, USA). The Au NWI injection was performed by a conventional microinjection system (Sutter instrument, USA) mounted on a Leica micromanipulator.