br Declaration of interest br Ethical

Declaration of interest
Ethical approval
Informed consent
Acknowledgments The present study was funded by the Institute Of Medical Genetics College Of Life Science, Sichuan University, China.
Introduction With the advancement of technologies, laboratory medicine and the need of routine biochemical analysis are changing. Mindray BS-2000M clinical chemistry system (BS-2000M1), recently launched by Shenzhen Mindray Bio-medical Electronics Co., Ltd. (Mindray, Shenzhen, China), is among the few automatic chemistry systems that can run at a speed of over 2000 tests/h worldwide. BS-2000M is a multi-parametric automated clinical chemistry system. It has 67 online channels, and the reaction carousel is composed of two layers of glass curettes (inner and outer carousels). To find out whether the test results detected by this bottle systems were consistent with those by Roche automatic biochemical analyzer detection system used in our laboratory currently, the biochemical outcomes of sixteen parameters detected by the two different biochemical detection systems were assessed for alignment and bias according to the Clinical and Laboratory Standard Institute (CLSI) protocol EP9-A2. This study was intended to compare the comparability of results from the two test systems and their clinical acceptability.
Materials and methods
Results
Discussion
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Author contributions
Acknowledgement We received research support from the three grants from the Evaluation of Application of Biochemical Instruments in Basic level units (No. 2013BAI17B04), the General Program of China during the 12th Five-Year Plan Period (No. CWS11J205) and the Military Major Special Project during the 12th Five-year Plan Period (No. 424135S).
Atherosclerosis (As) is a chronic inflammatory disease characterized by lipid deposition, macrophage infiltration, atherosclerotic plaque formation with thickening of the arterial wall. Inflammation in the vessel wall plays an important role in the initiation, progression and end stage of atherosclerosis. Multiple signaling pathways are involved in the activation, release and pathogenesis of inflammatory factors. There is a common confluence in many signaling pathways that regulate inflammation: p62/SQSTM1. As a multifunctional ubiquitin-binding protein, p62 plays an important role in selective autophagy and cellular signal transduction. Recent studies have shown that p62 protein can regulate the pathophysiology of atherosclerosis. In this article, we will review the functions of p62 protein and its role in atherosclerosis. Structural features of p62/SQSTM1 p62 is a multifunctional cytoplasmic protein that is an important regulatory molecule linking ubiquitinated protein to autophagy. The p62 protein is named after its molecular weight of 62kDa. The human p62 protein gene is located on chromosome 5 with 8 exons and 440 amino acid residues. The amino acid sequence of p62 contains multiple domains with structural motifs, which can interact with other functional proteins to produce biological effects. These functional domains from the N to the C end include: The Phox and Bem1p (PB1) domain located at the N-terminus; the ZZ-type zinc finger domain that binds to receptor-interactingprotein (RIP); a domain that binds to the P38 protein; the TB domain that binds to tumor necrosis factor receptor-associated factor 6 (TRAF6); a motif domain with two nuclear localization signals (NLS) and a nuclear export signal (NES); the LIR domain that interacts with the microtubule-associated protein 1A/1B-light chain 3 (LC3) on the autophagosome membrane; PEST sequences consists of proline (P), glutamate (E), serine (S) and threonine (T) residues; KIR domain that interacts with the ubiquitin linker protein Kelch epichlorohydrin-related protein 1 (Kelch-like ECH-associated protein-1, Keap1); the UBA domain at the C-terminus that can link to the ubiquitination substrate. The multifunctional structure of p62 protein makes it the center of multiple signaling pathways and it is also involved in the maintenance and the regulation of basic functions of cells. Therefore, dysfunction of P62 can lead to the obstruction of many signaling pathways and abnormal aggregation of proteins, which can lead to the development of various diseases.