br Materials and methods br Author disclosure statement

Materials and methods
Author disclosure statement
Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, Director of the Assisted Conception Unit of Guy\'s and St Thomas\' NHS Foundation Trust and his staff for supporting the research program. We are especially indebted to Prof Peter Braude and to the patients who donated embryos.
Resource table: mutant iPS cell lines. Resource details
Materials and methods
Acknowledgments The work in the author\'s laboratory is currently supported by the grant from NIH (R01GM093335). XL and CS conceived the study. CM, LL, LX and XL performed the experiments. XL wrote the manuscript.
Resource table
Resource details
We generated KCL012 clinical grade hESC line following protocols, established previously (Ilic et al., 2012; Stephenson et al., 2012). The expression of the pluripotency markers was tested after freeze/thaw HG-9-91-01 (Fig. 3; Ilic et al., 2012). Differentiation potential into three germ layers was verified in vitro (Fig. 4; Ilic et al., 2012) and in vivo (Fig. 5).
Materials and methods
Author disclosure statement
Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, Director of the Assisted Conception Unit of Guy\'s and St Thomas\' NHS Foundation Trust and his staff for supporting the research program. We are especially indebted to Prof. Peter Braude and to the patients who donated embryos.
Resource table
Resource details
We generated KCL017 clinical grade hESC line following protocols, established previously (Ilic et al., 2012; Stephenson et al., 2012). The expression of the pluripotency markers was tested after freeze/thaw cycle. Differentiation potential into three germ layers was verified in vitro.
Materials and methods
Author disclosure statement
Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, Director of the Assisted Conception Unit of Guy\'s and St Thomas\' NHS Foundation Trust and his staff for supporting the research program. We are especially indebted to Prof. Peter Braude and to the patients who donated embryos.
Resource table
Resource details To generate BJNhem20-TetR cell line, we transfected BJNhem20 by microporation at 1100V, 30ms pulse width and 1 pulse number. A stable hESC line was generated after subjecting these transfected cells to puromycin selection for two weeks. Expression of pluripotent stem cell markers OCT4, SSEA3, TRA1-60 and TRA1-81 has been shown by immunostaining. Differentiation of BJNhem20-TetR to all the three germ layers was demonstrated by immunostaining for beta III tubulin (ectodermal), Brachyury (mesodermal) and AFP (endodermal). Karyotype was also checked in these cells and found to be normal.
Materials and methods
Verification of karyotype
Acknowledgements This project was funded by the Department of Biotechnology, Government of India (Grant no. BT/PR11115/MED/31/40/2008) and the UK India Education and Research Initiative (Grant no. SA07-0062) and the Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore.
Resource table
Resource details
We generated KCL027 research grade hESC line following protocols, established previously (Ilic et al., 2012; Stephenson et al., 2012). The expression of the pluripotency markers was tested after freeze/thaw cycle (Fig. 2; Jacquet et al., 2015). Differentiation potential into three germ layers was verified in vitro (Figs. 3 and 5; Jacquet et al., 2015) and in vivo (Fig. 4; Jacquet et al., 2015).
Materials and methods
Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, Director of the Assisted Conception Unit of Guy\'s and St Thomas\' NHS Foundation Trust and his staff for supporting the research program. We are especially indebted to Prof Peter Braude and to the patients who donated embryos.